Absence of Monotony and Strain Effects on Referees' Physical Performance During International Basketball Federation World Cup Basketball Competition.

PURPOSE: The study aimed to conduct a comparative analysis of physical performance indicators and assess the levels of performance monotony and strain experienced by basketball referees. METHODS: The study involved the participation of 12 basketball referees (mean age: 40.0 [4.9] y) affiliated with the International Basketball Federation. The investigation was carried out throughout 2 density tournaments, wherein the maximum heart rate, average heart rate, performance monotony, and performance strain were documented for 3 variables. RESULTS: The findings indicated significant variations in the mean heart rate, maximum heart rate, total distance monotony, total distance strain, the total number of sprints monotony, the total number of sprints strain, calories monotony, and calories strain (P < .05). Decreasing the density of elite-level basketball competitions has been observed to reduce the monotony and strain experienced by referees. However, this action does not increase motion distance or speed zones. CONCLUSIONS: The environmental stressors experienced by senior-level athletes (World Cup) differ from those encountered by younger athletes (World Cup Under 19). Further investigation is required to ascertain the potential effects of competition monotony and strain on decision-making processes and the overall quality of refereeing.

Exploring Coaches' Cognitive Perception of Football Training Tasks: A Questionnaire-Based Study and Comparison with a Framework Tool for Assessing Cognitive Demands in Small-Sided Games / Exploración de la percepción cognitiva de los entrenadores sobre las tareas de entrenamiento de fútbol: un estudio basado en cuestionarios y una comparación con una herramienta marco para evaluar las demandas cognitivas en juegos reducidos / Exploração da perceção cognitiva de tarefas de treino em futebol: Estudo baseado numquestionário e comparação com uma ferramenta para avaliar e comparar as exigências cognitivas em jogos reduzidos

La función cognitiva es crucial en entornos de rendimiento competitivo debido al tipo de tareas y carga de trabajo involucradas. Sin embargo, aún se necesita determinar cómo toda la información ambiental en un entorno competitivo afecta la carga cognitiva.Por lo tanto, el objetivo fue investigar la percepción cognitiva de los entrenadores sobre las tareas de entrenamiento de fútbol, mediante el uso de un cuestionario y comparar los resultados con una herramienta de marco para evaluar las demandascognitivas en juegos reducidos. Un total de 57 entrenadores de fútbol (a nivel nacional y regional) completaron un cuestionario para calificar las demandas cognitivas percibidas de doce situaciones diferentes de juegos en espacios reducidos. Las respuestas fueron hechas por cada entrenador a partir de un conjunto de alternativas proporcionadas por los autores utilizando una escala de uso de Likert de 10 puntos. La carga cognitiva durante los juegos reducidos se calculó mediante una ecuación que incluía el número de jugadores implicados en la tarea, la superficie ocupada por cada jugador y la duración del ejercicio. Los resultados del cuestionario mostraron que los entrenadores de nivel nacional presentaron resultados de correlación más altos, mostrando una alta concordancia entre la percepción de los entrenadores y los resultados de la carga cognitiva en comparación con los entrenadores de nivel regional. La relación establecida entre todas las variables mostró que la complejidad de las tareas aumenta utilizando formatos con mayor número de jugadores, áreas pequeñas y ejercicios de mayor duración. Por lo tanto, el marco de carga cognitiva puede ser una herramienta útil y sencilla para quelos entrenadores evalúen las demandas cognitivas durante las sesiones de entrenamiento.(AU)

Cognitive function is crucial in competitive performance environments due to the type of tasks and workload involved. Thus, the present study aimed to investigate coaches’ cognitive perception of football training tasks, through the use of a questionnaire and compare the results with a framework tool to assess cognitive demands in small-sided games. A total of 57 football coaches (national and regional levels) filled out a questionnaire to rate the perceived cognitive demands of twelve different small-sidedgame situations. The answers were made by each coach from a set of alternatives supplied by the authors using a 10-point Likert use scale. The cognitive load during small-sided games was calculated using an equation that included the number of players involved in the task, the surface area occupied by eachplayer, and the duration of the exercise. The questionnaire results have shown that national-level coaches presented higher correlation results showing a high agreement between coaches' perception and the cognitive load results when compared to regional-level coaches. The relationship established between all variables showed that the complexity of tasks increases using formats with a larger number of players, small areas, and bigger duration drills. Thus, the cognitive load framework may be a useful and simple tool to coaches assess the cognitive demands during training sessions.(AU)

A função cognitiva é crucial em ambientes de desempenho competitivo devido ao tipo de tarefas e carga de trabalho envolvidas. No entanto, ainda precisa ser determinado como todas as informações ambientais em em contexto competitivo afetam a carga cognitiva. Assim, o objetivo deste estudofoi investigar a perceção cognitiva de treinadores sobre tarefas de treino de futebol, através de um questionário e comparando os resultados com um instrumento para avaliar as exigências cognitivas durante jogos reduzidos.Um total de 57 treinadores de futebol (nível nacional e regional) preencheram um questionário para avaliar as exigências cognitivas de doze situações diferentes jogos-reduzido. As respostas foram dadas por cada treinador a partir de um conjunto de alternativas fornecidas pelos autores utilizando uma escala de Likert de 10 pontos. A carga cognitiva durante pequenos jogos foi calculada através de uma equação queincluía o número de jogadores envolvidos na tarefa, a área de superfície ocupada por cada jogadore a duração do exercício. Os resultados do questionário mostraram que os treinadores de nível nacional apresentaram resultados de correlação mais elevados, mostrando uma alta concordância entre a perceção dos treinadores e os resultados de carga cognitiva em comparação com os treinadores de nível regional. A relação estabelecida entre todas as variáveis mostrou que a complexidade das tarefas aumenta utilizandoformatos com maior número de jogadores, áreas pequenas e exercícios de maior duração. Portanto, esta ferramenta para avaliar a carga cognitiva pode ser uma forma útil e simples para os treinadores avaliarem as exigências cognitivas durante as sessões de treino.(AU)

Tungsten oxide supported on copper ferrite: a novel magnetic acid heterogeneous catalyst for biodiesel production from low quality feedstock.

This study aims to synthesize a WO3/CuFe2O4 catalyst through a wet impregnation method and use it as a new magnetic acid catalyst in the transesterification process of waste cooking oil (WCO). The results of the characterization by XRD, FTIR, SEM, EDS, TG/DTG, VSM and Surface Acidity showed that the obtained bifunctional catalyst has been successfully synthesized. The study of the reaction parameters, such as reaction temperature (140-180 °C), reaction time (1-5 h), molar ratio MeOH : oil (25 : 1-45 : 1) and catalyst loading (2-10% m m-1) was performed in the conversion of WCO into biodiesel via transesterification. The reactional behavior showed the following optimal reaction conditions: reaction temperature of 180 °C, reaction time of 3 h, molar ratio MeOH : oil of 45 : 1 and catalyst loading of 6%. Based on the results, biodiesel with a maximum ester content of 95.2% was obtained using the WO3/CuFe2O4 magnetic catalyst under the optimal reaction conditions. The magnetic catalyst showed excellent catalytic and magnetic performance and it was applied in five reaction cycles with ester content above 80%. Biodiesel properties were found in accordance with ASTM limits. This research provided the development of a stable and reusable WO3/CuFe2O4 bifunctional catalyst for potential application in biodiesel production.

Muscle Oxygenation Measured with Near-Infrared Spectroscopy Following Different Intermittent Training Protocols in a World-Class Kayaker-A Case Study.

Training elite kayakers at a distance of 1000 m is associated with aerobic and anaerobic metabolism, while intermittent training, in a variety of forms, is one of the effective ways to improve cardiorespiratory and metabolic function. Thus, this study aimed to investigate muscle oxygenation responses during repetition training (RT), interval training (IT), and sprint interval training (SIT). Near-infrared spectroscopy (NIRS) monitors were placed on the latissimus dorsi (LD), pectoralis major (PM), and vastus lateralis (VL) of a world-class kayaker during their preparatory period. The intensity of work, relief, and recovery intervals were the independent variables that were manipulated using three different training protocols. The inferential analysis between intermittent training protocols showed significant differences for all variables except total the hemoglobin (tHb) index in LD during bout 2 (F = 2.83, p = 0.1, ηp2 = 0.205); bout 3 (F = 2.7, p = 0.125, ηp2 = 0.193); bout 4 (F = 1.8, p = 0.202, ηp2 = 0.141); and bout 6 (F = 1.1, p = 0.327, ηp2 = 0.092). During the rest bouts, all training protocols showed significant differences for all variables except muscle oxygen saturation (SmO2) in the VL during bout 5 (F = 4.4, p = 0.053, ηp2 = 0.286) and tHb in VL during bout 1 (F = 2.28, p = 0.132, ηp2 = 0.172); bout 2 (F = 0.564, p = 0.561, ηp2 = 0.049); bout 3 (F = 1.752, p = 0.205, ηp2 = 0.137); bout 4 (F = 1.216, p = 0.301, ηp2 = 0.1); and bout 6 (F = 4.146, p = 0.053, ηp2 = 0.274). The comparison between IT protocols RT and SIT presented similar results. All variables presented higher values during SIT, except HR results. Finally, the comparison between IT and SIT showed significant differences in several variables, and a clear trend was identified. The results of this study suggest that the application of different intermittent exercise protocols promotes distinct and significant changes in the peripheral effect of muscle oxygenation in response to training stimuli and may be internal predictors of hemodynamic and metabolic changes.

Exploring the impact of the COVID-19 pandemic in Euroleague Basketball.

The aim of this study was to understand how training and playing conditions during the COVID-19 pandemic affected the performance of Euroleague Basketball players. Using a non-participant observation analysis, the study compared the seasons before the lockdown (2018-2019 and 2019-2020; pre-pandemic) with the season after restart (2020-2021; pandemic). Paired t-tests and Wilcoxon tests were applied for variables with normal and non-normal distributions, respectively. The results revealed significant changes (p < 0.05) in several offensive and defensive performance-related variables during pandemic times (without attendance): free throw attempts, free throw percentage, turnovers, three-point attempt rate, fouls (small effect sizes, ESs), points, and possessions (trivial ES). The pre-pandemic HA (70%) significantly decreased after the lockdown, with games played with no crowd (∼51%; p = 0.018, large ES). The one-sample t-test showed that the HA after the COVID-19 interruption was not significantly greater than 50%, indicating that the HA did not endure during the pandemic condition. Although significant differences between home and away teams were found for most performance-related variables (excepting turnovers) in both pre-pandemic and pandemic conditions, variations of the relative HA were only significant for free throw attempts (large ES), points (medium ES), and turnovers (medium ES). The results of this study showed that performance variables were affected by the COVID-19 lockdown. Thus, these findings may help coaches, players, and referees to counteract unwanted competitive events and improve their overall performance, regardless of the contextual/situational circumstances encountered.

Effects of small-sided games vs. simulated match training on physical performance of youth female handball players.

The aim of this study was to compare the effects of different Small-Sided games (SSG) formats and simulated match handball training (SMHT) on handball player's physical performance and game activity profile. Twenty-four youth female handball players (age: 16.2 ± 1.5 years) participated in this study. The study was conducted during the first part of the competitive handball season and lasted for 10 weeks with 2 sessions per week in non-consecutive days: 1 week of pretesting, 8 weeks of specific training, and 1 week of post-testing. A two-group parallel randomized, pre- to post-test design was used to compare 2 different training groups: SSG training group (n = 12) and SMHT group (n = 12). The results showed larger improvements in drop jump height, jump power, absolute and relative anaerobic alactic power and 10 m sprint performances following the SSG training compared with the SMHT (p<0.05, ηp2 = ranging from 0.219 to 0.368). Game performance characteristics showed significant effect in SSD training in average sprint distance, total number of sprints and time between sprints (p<0.05, ηp2 = ranging from 0.08 to 0.292). The results of this study show that handball SSGs represent an adequate in-season strategy to promote a wide range of physical adaptations with improvements in running and jumping performance. This represents important information for coaches, since SSGs develop handball players' physical profiles while replicating tactical and technical features of the game. Nevertheless, simulated match training may be judiciously used to improve players' aerobic performance.

Physiological Responses and Technical-Tactical Performance of Youth Basketball Players: A Brief Comparison between 3x3 and 5x5 Basketball.

This study aims to examine youth players' physiological responses and technical-tactical performance when playing simulated 3x3 and 5x5 basketball games. Fifteen well-trained male basketball players (16.6 ± 0.2 years old) participated in scrimmage basketball games under two different conditions: 3x3 (half-court) and 5x5 (full-court). The players' heart rate, muscle oxygen saturation and total hemoglobin data were collected and computed to describe physiological responses, while video analysis was used to characterize their technical-tactical performance. A Bayesian one-way analysis of variance (ANOVA) was used to quantify the predictive influence of both game conditions on the physiological and the technical-tactical variables. The results indicated that different game conditions influenced the players' physiological responses slightly, as only hemoglobin sample entropy increased between the 3x3 and 5x5 game scenarios. Conversely, statistical differences in most of the technical-tactical variables were moderate and decisive in favour of the game condition model. Overall, this study emphasizes that playing 3x3 and 5x5 basketball games lead to relatively negligible differences in the players' physiological response but pronounced variations in their technical-tactical performance. Therefore, important implications may be drawn to the applied field as the specificity of technical-tactical adaptations when playing 3x3 or 5x5 formats should be considered by basketball coaches to better design the training sessions for players that fall within our sample age category.

Mining wastes to improve bitumen performances: An example of circular economy.

HYPOTHESIS: Inorganic small particles stemming from mineral extraction (i.e. mining waste) could be used as additive for road paving applications to improve bitumen mechanical properties. Such an approach is expected to increase bitumen life-cycle cutting costs connected to their preparation and to reduce environmental issues. Experiment: Bitumens containing various amounts (up to 10% w/w) of filler made of mining tailings fine powder were characterized by means of oscillatory rheometry focusing on the effect of the filler content, temperature and filler milling time. FINDINGS: (i) Superior resistance to stress, rutting, and fatigue were shown by the filler-containing mixtures. In addition, higher durability was observed for the filler concentration of 10% w/w. These effects were interpreted on the grounds of the physico-chemical interactions between the bitumen and the inorganic filler suggesting important utilizations. (ii) The present research points towards a circular economy path. Particularly, this study demonstrates how an abundant and potentially harmful waste can be converted into a high value-added component for road paving. Furthermore, increased durability of bitumen is beneficial in both economic and environmental terms.

Repeated Sprint Ability in Elite Basketball Players: The Effects of 10 × 30 m Vs. 20 × 15 m Exercise Protocols on Physiological Variables and Sprint Performance.

Team sports players are required to perform repeated bouts of short-term high-intensity actions during the games. The present study aimed to examine the effects of a novel repeated sprint ability protocol (20×15 m) and compare it with the impact of a more traditional repeated sprint ability protocol (10×30 m). Twelve male elite Lithuanian basketball players (age 21.0 ± 2.0 y, body height 1.90 ± 0,07 m, body mass 86.2 ± 5.8 kg and training experience 12.0 ± 1.9 y) competing in the Lithuanian National Basketball Championship participated in this study. Participants completed three bouts of each repeated sprint protocol interspersed with 5 minutes of recovery. Results showed that the 20×15 m protocol caused a significant decrease in total sprint time (most likely; mean changes (%) with ± 90% of confidence limits, -9.4%; ± 0.7%) and a large decrease in blood lactate (most likely, -39.2%; ±12.8%) compared to the 10×30 m protocol. Despite small differences, the fatigue index presented a similar trend (possibly decrease, -23.7%; ± 38.8%). The exercise heart rate showed a very similar trend with trivial differences between the two protocols. The 20×15 m protocol presented a lower heart rate during recovery with small magnitude. Overall, the present study showed that the 20×15 m protocol seemed to be more representative of the specific basketball demands. Coaches should be aware that RSA training during the in-season may be an adequate stimulus to improve high-intensity runs and muscle power in high-level players.

Different Marks in the Pitch Constraint Youth Players' Performances During Football Small-sided Games.

Purpose: This study aimed to compare players' performances when manipulating the external markings of the pitch during football small-sided games. Methods: Ten under-15 players performed a 5-a-side (plus goalkeepers) under three conditions: (i) Lines, the game was played in a pitch in which the external boundaries were painted with full lines; (ii) Dashed, the game was played in a pitch in which the external boundaries were painted with dashed lines; (iii) Corners, the game was played in a pitch were the external boundaries were delimited by one marker at each pitch corner. Players' positional data was used to compute tactical and time-motion variables. Also, technical analysis was comprised using video footage. Results: Results showed similar tactical, physical and technical performances between the Lines and Dashed conditions. In contrast, the Lines condition showed small higher effects than Corners scenario in the time spent synchronized in longitudinal and lateral displacements, game pace, total distance covered, distance covered while jogging, number successful dribbles and shots on target. The Lines scenario has also revealed a lower effective playing space, distance covered at walking and running and a lower number of passes (small effects) compared to Corners. Conclusions: These results highlight that these changes in informational perception constraints modify players movement behavior. Accordingly, pitches with more visible boundaries were likely to decrease team dispersion, which may optimize team synchrony and technical performances, while decreasing the distance covered at higher speeds. Coaches may use this information to modify the types of pitch external boundaries markings, exposing the players to different environmental information.

Validity and reliability of Gyko Sport for the measurement of barbell velocity on the bench-press exercise.

BACKGROUND: The aim of this study was to examine the validity and reliability of the novel inertial measurement unit (named: Gyko Sport) for the measurement of barbell concentric velocity on the bench-press exercise. METHODS: Ten under-16 (U16) basketball players performed 2 repetitions of the bench press exercise with 6 different loads (40, 50, 60, 70, 80 and 90% of one repetition maximum [1-RM]; N.=120 repetitions). Mean velocity for each repetition was simultaneously recorded using the Gyko Sport and SmartCoach linear transducer. RESULTS: Results showed a very large correlation between the SmartCoach and Gyko Sport (r=0.79; standard error of estimate [SEE]=0.18 m/s). Furthermore, there was a very high agreement between both devices for the measurement of the mean velocity (intraclass correlation coefficient [ICC]=0.774; Cronbach's alpha [α]=0.872; Bland-Altman Plots [R2]=0.070). The Gyko Sport registered similar, but slightly higher values than the SmartCoach (P=0.103; mean difference 0.075±0.05 m/s). CONCLUSIONS: The results support the use of the Gyko Sport as an affordable, portable, and suitable wearable device to measure mean velocity on bench press exercise.

Exploring how playing football with different age groups affects tactical behaviour and physical performance.

The study aimed to compare footballers' performances when playing with teammates and opponents from the same age group with performances when playing with teammates and opponents of different age groups. Three football matches were played: i) under-15 (U15) players played with each other; ii) under-17 (U17) players played with each other; and iii) players under the age of 15 and 17 played with each other in two equivalent mixed age teams. The players' physical performance was measured using the distances covered at different speed categories and tactical behaviour was assessed using several positioning-derived variables. The results showed that, when playing in the mixed age condition, the U15 players increased the distance covered in sprinting intensity (18.1%; ±21.1%) and the U17 players increased the distance covered in jogging zones (6.8%; ±6.5%). The intra-team movement synchronization in longitudinal and lateral displacements was higher when U15 players confronted peers of the same age, in the first half (-13.4%; ±2.0%, -20.3%; ±5.7% respectively), and when U17 players confronting the mixed group, in both halves (-16.9%; ±2.5%, 9.8%; ±4.0% and 7.9%; ±5.7%, 10.6% ±4.4%, respectively). The differences between age groups and the mixed condition may be connected with the level of players' tactical expertise and adaptive positioning according to the dynamic environmental information. In general, these results suggest that mixing the age groups may be useful to promote a wider range of training session stimuli in these young football players.

Accuracy of a Basketball Indoor Tracking System Based on Standard Bluetooth Low Energy Channels (NBN23®).

The present study aims to identify the accuracy of the NBN23® system, an indoor tracking system based on radio-frequency and standard Bluetooth Low Energy channels. Twelve capture tags were attached to a custom cart with fixed distances of 0.5, 1.0, 1.5, and 1.8 m. The cart was pushed along a predetermined course following the lines of a standard dimensions Basketball court. The course was performed at low speed (<10.0 km/h), medium speed (>10.0 km/h and <20.0 km/h) and high speed (>20.0 km/h). Root mean square error (RMSE) and percentage of variance accounted for (%VAF) were used as accuracy measures. The obtained data showed acceptable accuracy results for both RMSE and %VAF, despite the expected degree of error in position measurement at higher speeds. The RMSE for all the distances and velocities presented an average absolute error of 0.30 ± 0.13 cm with 90.61 ± 8.34 of %VAF, in line with most available systems, and considered acceptable for indoor sports. The processing of data with filter correction seemed to reduce the noise and promote a lower relative error, increasing the %VAF for each measured distance. Research using positional-derived variables in Basketball is still very scarce; thus, this independent test of the NBN23® tracking system provides accuracy details and opens up opportunities to develop new performance indicators that help to optimize training adaptations and performance.

Basketball Game-Related Statistics that Discriminate Between European Players Competing in the NBA and In the Euroleague.

This study aimed to identify the game-related statistics that discriminated between Euroleague basketball players and European basketball players playing in the NBA, when competing in the same event (EuroBasket 2015). There was a total of 78 matches played by 24 teams in two groups of analysis: NBA, participants in the European Championship who played in the NBA season of 2014-2015 (n = 26); Euroleague, participants in the European Championship who played in the Euroleague season of 2014-2015 (n = 82). The players' performance variables were normalized to the time they spent on the court. To identify which variables best discriminated between the NBA and the Euroleague performance profiles, a descriptive discriminant analysis was conducted. Structure coefficients (SC) from the matrix greater than |0.30| were interpreted as meaningful contributors to discriminating between the groups. The results revealed a significant function (p = 0.008, canonical correlation of 0.51, Λ = 0.74, reclassification = 84.2%) and substantial performance differences in game-related statistics much related to the influence of body size (body height and mass), such as two-point field goals made (SC = 0.42) and missed (SC = 0.40), free-throws made (SC = 0.55), defensive rebounds (SC = 0.62), blocks (SC = 0.48) and suffered fouls (SC = 0.34). No differences were found at the level of game-related statistics indirectly related to perception, such as assists, turnovers or steals. Also, the greater body size in NBA players was likely related to higher variability in performance, thus, being an important topic for coaches and recruiters to analyse.

Typical weekly workload of under 15, under 17, and under 19 elite Portuguese football players.

This study aims to describe the time-motion and physiological performance profiles of footballers whose ages are under 15 (U15), under 17 (U17), and under 19 (U19) during a typical week of a competitive season. A total of 151 elite Portuguese players U15 (age 14.0 ± 0.2; n = 56), U17 (age 15.8 ± 0.4; n = 66), and U19 (age 17.8 ± 0.6; n = 19) were monitored during 33 training sessions (TSs) (U15 n = 12; U17 n = 11; and U19 n = 10 TSs). The TS data were captured at 15 Hz by global positioning systems devices and divided into post-match (session after the match), prematch (session before the match), and middle week (average of remaining sessions). The U15 middle week showed a higher number of sprints, distance covered in intermediate speed zones, and time spent above 90% HRmax, while the prematch presented a higher distance covered above 18 km · h(-1) and time spent below 75% HRmax. In U17, both prematch and post-match data presented lower values than middle-week data in most of the variables. The post-match data in U19 presented higher values of distance covered above 13 km · h(-1), body impacts above 10 G, and time spent above 85% HRmax, while middle week showed higher values in body impacts in most of the zones. In addition, the prematch data presented 35% to 100% less values than the middle-week data. Understanding the weekly workload variations according to the competition and the developmental ages of the players can contribute to optimising short- and mid-term planning.

Effect of player position on movement behaviour, physical and physiological performances during an 11-a-side football game.

The aim of this study was to identify differences in time-motion, modified training impulse, body load and movement behaviour between defenders, midfielders and forwards, during an 11-a-side simulated football game. Twenty elite youth male footballers from the same squad participated in this study (age: 18.1 ± 0.7 years old, body mass: 70.5 ± 4.3 kg, height: 1.8 ± 0.3 m and playing experience: 9.4 ± 1.3 years). All data were collected using GPS units (SPI-Pro, GPSports, Canberra, Australia). The movement behaviour was measured with kinematic data, used to calculate position-specific centroids (defenders, midfielders and forwards), and processed with non-linear statistical procedures (approximate entropy normalised and relative phase). There were significant effects and interactions in all variables across the players' positions. The results showed that displacements of all players (defenders, midfielders and forwards) were nearer and more coordinated with their own position-specific centroids than with the other centroids. However, this coupling effect was stronger in midfield players and weaker in forwards. All players' dynamical positioning showed more irregularity when related to the forwards' centroid, as a consequence of their need to be less predictable when playing. The time-motion and physiological variables showed lower activity in forward players. Adding together, the results may contribute to a better understanding of players' specific performances and football complexity.

Chemical constituents and allelopathic and antioxidant activities of Alchorneopsis floribunda Müll. Arg. (Euphorbiaceae).

Chemical investigation of extracts from the stems and leaves of Alchorneopsis floribunda Müll. Arg., collected in the Amazon region, was performed. The main isolated compounds were triterpenes (α-amyrin, ß-amyrin, lupeol, betulin, betulinic acid, uvaol, erythrodiol and oleanolic acid) and phenolic acid derivatives from 4-hydroxybenzoic acid (gallic and protocatechuic acids and isocorilagin). In the germination assays, high inhibitory allelopathic effects of the extracts and isocorilagin were observed and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity of isocorilagin was higher than those of the standards used (Trolox and butylated hydroxyanisole). This is the first chemical study of the genus Alchorneopsis (Euphorbiaceae).

Prevalência e susceptibilidade aos antimicrobianos de Staphylococci isolados de vagina de ovelhas sadias / Prevalence and antimicrobial susceptibility of Staphylococci isolated from the vagina of healthy ewes

Os objetivos deste estudo foram: identificar as espécies de Staphylococcus sp. da vagina de ovelhas sem sinal de infecção e determinar sua susceptibilidade in vitro aos antimicrobianos. Swabs estéreis foram usados para coletar as amostras da vagina de 24 ovelhas da raça Santa Inês, do mesmo rebanho, classificadas como livres de doenças genitais após um histórico e exame físico detalhados. Procedimentos bacteriológicos padronizados, como por exemplo, o isolamento em meio de cultura seletivo e crescimento das amostras em aerobiose, foram utilizados para identificação dos isolados. Espécies de Staphylococcus coagulase-positivo (CoPS) representaram 60% dos isolados e foram significantemente mais resistentes do que as espécies de Staphylococcus coagulase-negativo. A resistência aos antibióticos foi frequentemente observada, e 66,6% dos isolados demonstraram resistência à pelo menos uma droga. Ciprofloxacina foi o agente antimicrobiano mais eficiente, não apresentando nenhuma cepa resistente, enquanto a Penicilina G foi a droga menos efetiva (40% de resistência). Esse estudo confirma a presença de amostras estafilocócicas na vagina de ovelhas, com predominância de CoPS que apresentaram resistência a diversos antibióticos testados. Este trabalho contribui para um melhor esclarecimento sobre o papel dos staphylococci na vagina das ovelhas e sua susceptibilidade aos antimicrobianos, colaborando para um tratamento de vaginite mais eficiente, que pode ser causada por este gênero bacteriano.

The aims of this study were identify the species of Staphylococcus sp. from the vagina of healthy ewes and determine their in vitro susceptibility to antibiotics. Sterile cotton swabs were used to collect samples from the vagina of 24 ewes. Standard bacteriological procedures were conducted. Coagulase-positive Staphylococcus species (COPS) represented 60% of the isolates and were significantly more resistant to antibiotics than coagulase-negative isolates. Resistance to antibiotics was frequently observed, and 66.6% of the isolates showed resistance to at least one tested drug. Ciprofloxacin was the most active antimicrobial agent (100%), while Penicillin G was the less effective (40% of resistance). This study confirms the presence of Staphylococcal isolates in the vagina of ewes, with predominance of CoPS isolates resistant to various antibiotics. This study contributes to a better knowledge about the role of Staphylococcus species in the ewe’s vagina and their antimicrobial susceptibility, collaborating for a better treatment of the vaginitis determined by these bacteria.